Glucagon Degrading Activity

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[Glucagon degrading activity].

To evaluate the effect of glucagon degrading activity (GDA) on radioimmunoassay (RIA) of glucagon, I measured GDAs in plasma, serum, lysed red blood cells (RBC), and suspension of mononuclear cells and granulocytes. Serum levels of GDA in patients with various diseases were also examined. 1. Serum GDA values in normal subjects (control) measured by TCA precipitation method were 4.6 +/- 2.2% (me...

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Antitumor activity of glucagon.

MATERIALS AND METHODS Ascites tumors were implanted intraperitoneally (I.P.) with 0.1 ml. of a 1:5 dilution of packed cells except for the L1210 leukemia, for which 0.5 ml. of a 10 -4 dilution of ascitic fluid was used. The P1534 leukemia was implanted with 0.5 ml. of a 10 -4 spleen suspension. Implants of all other tumors, approximately 1 cu. mm., were implanted subcutaneously by trocar. Insul...

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Metal ions affect insulin-degrading enzyme activity.

Insulin degradation is a finely tuned process that plays a major role in controlling insulin action and most evidence supports IDE (insulin-degrading enzyme) as the primary degradative agent. However, the biomolecular mechanisms involved in the interaction between IDE and its substrates are often obscure, rendering the specific enzyme activity quite difficult to target. On the other hand, biome...

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THE EFFECTS OF GLUCAGON, INSULIN AND S TEROID HORMONES ON PHOSPHATIDATE PHOSPHOHYDROLASE ACTIVITY IN RAT LIVERS

The effects of steroid hormones, glucagon and insulin on rat liver phosphatidate phosphohydrolase (PAP) activity were studied both in vitro and in vivo. Incubation of rat hepatocytes with each hormone showed that dehydroepiandrosterone (DHEA), progesterone and testosterone increase PAP activity by 44.6, 37 and 36.9%, respectively. Estradiol, however, decreased enzyme activity by 13.6% under...

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Lipolytic and adenyl-cyclase-st imulat ing activity of ?~-trinitrophenyl glucagon: comparison with other glucagon

It has recently been reported from one of our laboratories that the Nc~-tr ini trophenyI (TNP) derivat ive of glucagon stimulates hepatic g lycogenolys is w i th an in t r ins ic ac t i v i t y comparable to that of glucagon. This compound, however, has negligible abil i ty to stimulate ! lver adeny! cyciase ( i ) . The derivative is also a competit ive antagonist of the action of glucagon on l...

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ژورنال

عنوان ژورنال: Folia Endocrinologica Japonica

سال: 1989

ISSN: 0029-0661

DOI: 10.1507/endocrine1927.65.5_483